Composite

Part:BBa_K4323001:Design

Designed by: Jessica Marinelli, Kevin Lim, Chun Hin Wong   Group: iGEM22_UManitoba   (2022-09-30)


Composite part to express Aerolysin toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
    Illegal AgeI site found at 363
    Illegal AgeI site found at 1320
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We choose suitable promoters, RBS, and terminator to design this part. Between the RBS and the Coding region is a custom scar sequence (TACTAT)


Source

This composite part is synthesized and cloned by the 2022 UManitoba team.

References